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We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 μm × 100 μm-sized fields of view in <1 s using 116,000 such doughnuts.

Original publication

DOI

10.1038/nmeth.2556

Type

Journal article

Journal

Nat Methods

Publication Date

08/2013

Volume

10

Pages

737 - 740

Keywords

Animals, Cell Line, Female, Humans, Male, Microscopy, Fluorescence, Nanotechnology, Neurons, Rats, Rats, Wistar