Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

This chapter presents the foundations of STED microscopy with a comparison to its generalization Resolft microscopy and to stochastic imaging methods (PALM, STORM, FPALM, and alike). The first section reviews the advantages of optical microscopy, explains the diffraction limit, and shows how the classical resolution limit was finally broken. It also reviews some of the achievements in super-resolution imaging. The second section explains in depth the principle of STED microscopy and highlights some special STED modalities like the use of continuous wave lasers, time-gating, fast imaging with up to 200 frames per second, and combination with fluorescence correlation spectroscopy. The third section treats some aspects of resolution in the presence of noise, especially in the scope of high-resolution imaging. © 2014 Springer Science+Business Media, LLC.

Original publication

DOI

10.1007/978-1-62703-983-3-3

Type

Journal article

Journal

Neuromethods

Publication Date

01/01/2014

Volume

86

Pages

41 - 71