Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Although DNA vaccines have been shown to elicit potent immune responses in animal models, initial clinical trials in humans have been disappointing, highlighting a need to optimize their immunogenicity. Naked DNA vaccines are usually administered either i.m. or intradermally. The current study shows that immunization with naked DNA by direct injection into a peripheral lymph node enhances immunogenicity by 100- to 1,000-fold, inducing strong and biologically relevant CD8(+) cytotoxic T lymphocyte responses. Because injection directly into a lymph node is a rapid and easy procedure in humans, these results have important clinical implications for DNA vaccination.

Original publication

DOI

10.1073/pnas.051630798

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

13/03/2001

Volume

98

Pages

3299 - 3303

Keywords

Animals, Antigens, Viral, Cell Line, DNA, Viral, Epitopes, T-Lymphocyte, Glycoproteins, Homeodomain Proteins, Immunodominant Epitopes, Injections, Intralymphatic, Lymphocytic Choriomeningitis, Lymphocytic choriomeningitis virus, Mice, Mice, Inbred C57BL, Mice, Knockout, Peptide Fragments, Plasmids, T-Lymphocytes, Cytotoxic, Vaccination, Vaccines, DNA, Viral Proteins, Viral Vaccines