Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Mechanisms by which T and B lymphocytes co-operate in the in vitro secondary antibody response to trinitrophenyl (TNP)-conjugated soluble protein antigens were investigated. The generation of antibody responses was analyzed when haptenic and carrier determinants were either linked or non-linked. Ability to co-operate through each of these mechanisms was influenced by the experimental conditions employed, particularly the mode of preparation of the T cells and the antigen concentration used. Nylon wool filtration of T cells may deplete a T helper population involved in non-linked recognition interactions. High antigen concentrations favour the non-linked form of interaction whereas low antigen concentrations favour linked recognition interaction. These data suggest that at least two distinct co-operative mechanisms co-exist. However, experimental conditions can be defined under which either one mechanism predominates or where more than one mechanism is relevant.

Type

Journal article

Journal

Immunology

Publication Date

02/1984

Volume

51

Pages

343 - 350

Keywords

Animals, Antibody Formation, Antigens, B-Lymphocytes, Cells, Cultured, Concanavalin A, Female, Hemocyanins, Lymphocyte Cooperation, Male, Mice, Mice, Inbred CBA, Spleen, T-Lymphocytes, Trinitrobenzenes