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The bHLH transcription factor, Hand1 has been implicated in cardiac looping in the mouse, however its function in the developing heart remains unknown. To investigate the mechanism(s) through which Hand1 might function, we screened for potential downstream target genes using representational difference analysis. Thymosin beta4 was found to be down-regulated whereas cystatin C and alphaCA were up-regulated in Hand1-null embryoid bodies. Whole-mount in situ hybridisation on wild type embryos (E8.0-E10.5) and Hand1 homozygous-mutant embryos (E8.0) confirmed co-expression of the putative targets with Hand1 in the heart and their aberrant expression in a Hand1-null background.

Type

Journal article

Journal

Mech Dev

Publication Date

12/2002

Volume

119 Suppl 1

Pages

S65 - S71

Keywords

Animals, Basic Helix-Loop-Helix Transcription Factors, Gene Expression Regulation, Developmental, Heart, In Situ Hybridization, Morphogenesis, Transcription Factors