Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

In the steady state, dendritic cells (DCs) in the lymph node induce T cell tolerance to self antigens. Innate signals trigger the maturation of tissue DCs, which migrate into lymph nodes and activate T cells. To examine DCs in vivo, we produced transgenic mice whose DCs expressed enhanced yellow fluorescent protein. Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes. Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells.

Original publication

DOI

10.1038/ni1139

Type

Journal article

Journal

Nat Immunol

Publication Date

12/2004

Volume

5

Pages

1243 - 1250

Keywords

Animals, CD11c Antigen, Cell Communication, Cell Differentiation, Cell Movement, Dendritic Cells, Lymph Nodes, Mice, Mice, Transgenic, Microscopy, Confocal, Microscopy, Fluorescence, Recombinant Proteins, T-Lymphocytes