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Authors: Alsoussi et al. 

Journal/ Pre-Print:Journal of Immunology  

Key Words: Immunology, Antibody 

Research Highlights

  1. Generation and characterization of a panel of murine monoclonal antibodies (mAbs) directed against the receptor-binding domain (RBD) 

  1. Antibody 2B04 is shown to both neutralize SARS-CoV-2 very potently in vitro and in vivo in a mouse model. 

Summary

This study aims to identify potent neutralising mAb against SARS-CoV2 RBD for the purpose of generating antibodies for therapy.  Mice were immunized with recombinant SARS-CoV-2 RBD and adjuvant and boosted 14 days later twice with recombinant SARS-CoV-2 spike (S) protein at a 10-day interval. 5 days after the final booster immunization potent neutralizing serum activity was measured against recombinant S or RBD protein and anti-SARS-CoV-2 antibodies were isolated and cloned from sorted plasma blasts. Five isolated antibodies were shown to display strong neutralizing activity against SARS-CoV-2 in vitro, amongst which the antibody clone 2B04 showed the most potent activity. In vivo protective activity of 2B04 was tested using a mouse model of SARS-CoV-2 infection in which hACE2 is transiently expressed via a nonreplicating adenoviral vector. 2B04 was shown to limit disease and reduce viral dissemination. 

Impact for SARS-CoV2/COVID19 research efforts  

Inhibit of SARS-CoV2/COVID19 transmission 

Treat of SARS-CoV2/COVID19 positive individuals 

Study Type 

  • In vitro study 

  • In vivo study (e.g. mouse, NHP) 

Strengths and limitations of the paper 

Novelty: Potently neutralizing anti-SARS-CoV-2 antibodies have been isolated previously 

Standing in the field:There is already a couple of papers published reporting similar results with other mAb. 

Appropriate statistics: Yes 

Viral model used:They use wild-type SARS-CoV-2 for their in vitro assay and the strain 2019 n-CoV/USA_WA1/2020 for their in vivo infection  

Translatability:2B04 could be developed as a therapy 

Main limitations: They generate mouse antibodies and show in vivo efficacy only in mice