A shift towards an immature myeloid profile in peripheral blood of critically ill COVID-19 patients
bioinformatics immunology/immunity molecular biology
Authors:Keiko Taniguchi-Ponciano et al.
Link to paper: https://www.researchsquare.com/article/rs-38042/v1
Tags: Bioinformatics, Immunology/Immunity, Molecular biology
Circulating immune cell profile of severe COVID-19 patients is skewed towards immature myeloid populations.
Circulating immune cells from 5 severe COVID-19 patients undergoing mechanical ventilation were used for single cell RNAseq. The cells were compared to an unspecified cohort of healthy controls. The cells were devided into different immune populations according to published markers. The authors see an increase in myeloid populations in COVID-19 patients compared to control, in particular immature myeloid cells such as band neutrophils, metamyelocytes and promyelocytes-myelocytes. Due to the poor resolution of the figures it is difficult to confirm the author’s statements.
Impact for SARS-CoV2/COVID19 research efforts
Understand the immune response to SARS-CoV2/COVID19
The authors show an increase in immature myelocyte populations in severe cases
Strengths and limitations of the paper
Novelty: This is not the first study of this type. The authors confirm the skew towards myeloid cells compared to lymphoid cells in COVID-19 patients and also show how the myeloid cells have an immature phenotype.
Standing in the field:the results confirm previous findings and it is known that severe viral infection can drive increase of immature myeloid cell populations
Viral model used:N/A
The resolution of the figures does not allow the comprehension of the content
The figure legend is non-existent
There is no information about the control patients
It is not clear how the pooling/barcoding has been done (before or after several patients were pooled)
Small number of patients with different pre-existing conditions and one with another bacterial infection
No information about statistical methods