Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Authors:Dobaño et al. 

Journal/ Pre-Print:BioRxiv 

Tags: Clinical/ Diagnostics 

Research Highlights 

  1. Developed a sensitive multiplex assay using quantitative suspension array technology (qSAT)s which included RBD, S1, S2, S, M and N SARS-CoV-2 antigens 

  1. Observed some cross-reactivity of negative control (pre-pandemic) sera for M, S2 and N SARS-CoV-2 antigens 

Summary 

The authors developed a time-optimised qSAT assay based on a Luminex platform which measured antibodies IgG, IgM, and IgA against SARS-CoV-2 antigens RBD, S1, S2, S (spikeprotein and its components), M (membrane) and N (nucleoprotein). The authors use of multiplexing with different combinations of antibody isotype and antigen increased specificity and sensitivity. High specificity and sensitivity was observed against 128 pre-pandemic plasma negative controls and 115 positive control plasmas. Cross-reactivity to M, S2 and N antigens was observed for some negative controls. The authors note there is further work to validate this potentially useful tool, which could then be applied to vaccine development and/or antibody studies. 

Impact for SARS-CoV2/COVID19 research efforts 

Develop diagnostic tools for SARS-CoV2/COVID19 

Others: Develop a multiplexed antibody detection tool for application of post-vaccine efficacy and post-infection antibody studies 

Study Type  

  • In vitro study 

Strengths and limitations of the paper 

Novelty: Developed a new multiplexing tool to study antibody responses to multiple antigens of SARS-CoV-2  

Standing in the field: Builds on other serological assays, but lacks supporting informationfrom conventional ELISAs          

Appropriate statistics:Good statistics with specificities and sensitivities. However, averages with error bars would have made comparative data clearer 

Viral model used:SARS-CoV-2 antigens 

Translatability:Possible research tool for vaccine development and/or antibody studies 

Main limitations: 

  • Volume of sample used per test compared to volume used in an ELISA is not clear 

  • No comparison of multiplex test with ELISA to show comparability 

  • Would have been nice to have had a comparison of antibody-antigen detection over the four different time points from onset of symptoms 

  • M protein purity described as being “not high”, would have been better for robustness of assay to have been purer 

  • Short times described as a benefit of the assay. However, no mention of time required to carry out assay 

  • No conclusion reached on which antibody-antigen combinations had the most potential