Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Authors: Jon Arizti-Sanz et al

Link to paper:

Journal/ Pre-Print: biorxchiv

Key Words: Cas13a SHINE

Research Highlights 

1. Single-step detection of SASR-CoV-2 RNA with LOD 10cp/µL using fluorescence as read-out (named SHERLOCK)

2. App that uses camera of mobile phone to interpret the fluorescence read-out

3. SHERLOCK reaction optimized to detect SARS-CoV-2 RNA extracted with Heating Unextracted Diagnostic Samples to Obliterate Nucleases (HUDSON).


The authors have optimized a Cas13-based detection method for SARS-CoV-2 RNA extracted by Heating Unextracted Diagnostic Samples to Obliterate Nucleases (HUDSON) from patient saliva and nasopharyngeal swabs. Limit of detection is 10 copies (cp)/µL when using fluorescence as read-out (comparable sensitivity to RT-PCR), that can be combined with an app that provides a binary result using a picture of the tube taken with a mobile phone camera.

Impact for SARS-CoV2/COVID19 research efforts

Develop diagnostic tools for SARS-CoV2/COVID19

Study Type 

· In vitro study

Strengths and limitations of the paper

Novelty: Optimized for RNA extracted with HUDSON along with the app to capture the result, and generates a novel dataset.

Standing in the field: LOD of 10cp/µL is the required level of detection. Multiple Cas-based tests have been developed, but this one is optimized for use with HUDSON RNA extracts.

Appropriate statistics: yes

Viral model used: SARS-CoV-2 viral seedstocks (no reference)

Translatability: Ready to be used in the field

Main limitations: Not a novel method, simply an optimized version of SHERLOCK and HUDSON.

Although the developed method is faster and does not require specialized lab equipment, the sensitivity is slightly less that RT-PCR when comparing in NP swabs.

Authors refer to Fig 2I in text, but that fig does not exist.