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The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was used to produce a full thickness atlas of intestinal cells across human development. This protocol can also be adapted to adult endoscopy and surgical specimens. For details on the use of this protocol, please refer to Fawkner-Corbett et al. (2021).

Original publication

DOI

10.1016/j.xpro.2021.100890

Type

Journal article

Journal

STAR Protoc

Publication Date

17/12/2021

Volume

2

Keywords

Cell Biology, Cell isolation, Developmental biology, Flow Cytometry/Mass Cytometry, Single Cell