Interaction between ERAP1 and HLA-B27 in ankylosing spondylitis implicates peptide handling in the mechanism for HLA-B27 in disease susceptibility.

Evans DM., Spencer CCA., Pointon JJ., Su Z., Harvey D., Kochan G., Oppermann U., Dilthey A., Pirinen M., Stone MA., Appleton L., Moutsianas L., Leslie S., Wordsworth T., Kenna TJ., Karaderi T., Thomas GP., Ward MM., Weisman MH., Farrar C., Bradbury LA., Danoy P., Inman RD., Maksymowych W., Gladman D., Rahman P., Spondyloarthritis Research Consortium of Canada (SPARCC) None., Morgan A., Marzo-Ortega H., Bowness P., Gaffney K., Gaston JSH., Smith M., Bruges-Armas J., Couto A-R., Sorrentino R., Paladini F., Ferreira MA., Xu H., Liu Y., Jiang L., Lopez-Larrea C., Díaz-Peña R., López-Vázquez A., Zayats T., Band G., Bellenguez C., Blackburn H., Blackwell JM., Bramon E., Bumpstead SJ., Casas JP., Corvin A., Craddock N., Deloukas P., Dronov S., Duncanson A., Edkins S., Freeman C., Gillman M., Gray E., Gwilliam R., Hammond N., Hunt SE., Jankowski J., Jayakumar A., Langford C., Liddle J., Markus HS., Mathew CG., McCann OT., McCarthy MI., Palmer CNA., Peltonen L., Plomin R., Potter SC., Rautanen A., Ravindrarajah R., Ricketts M., Samani N., Sawcer SJ., Strange A., Trembath RC., Viswanathan AC., Waller M., Weston P., Whittaker P., Widaa S., Wood NW., McVean G., Reveille JD., Wordsworth BP., Brown MA., Donnelly P., Australo-Anglo-American Spondyloarthritis Consortium (TASC) None., Wellcome Trust Case Control Consortium 2 (WTCCC2) None.

Ankylosing spondylitis is a common form of inflammatory arthritis predominantly affecting the spine and pelvis that occurs in approximately 5 out of 1,000 adults of European descent. Here we report the identification of three variants in the RUNX3, LTBR-TNFRSF1A and IL12B regions convincingly associated with ankylosing spondylitis (P < 5 × 10(-8) in the combined discovery and replication datasets) and a further four loci at PTGER4, TBKBP1, ANTXR2 and CARD9 that show strong association across all our datasets (P < 5 × 10(-6) overall, with support in each of the three datasets studied). We also show that polymorphisms of ERAP1, which encodes an endoplasmic reticulum aminopeptidase involved in peptide trimming before HLA class I presentation, only affect ankylosing spondylitis risk in HLA-B27-positive individuals. These findings provide strong evidence that HLA-B27 operates in ankylosing spondylitis through a mechanism involving aberrant processing of antigenic peptides.

DOI

10.1038/ng.873

Type

Journal article

Journal

Nat Genet

Publication Date

10/07/2011

Volume

43

Pages

761 - 767

Keywords

Aminopeptidases, CARD Signaling Adaptor Proteins, CD8-Positive T-Lymphocytes, Case-Control Studies, Core Binding Factor Alpha 3 Subunit, Disease Susceptibility, European Continental Ancestry Group, Genome-Wide Association Study, HLA-B27 Antigen, Humans, Interleukin-12 Subunit p40, Latent TGF-beta Binding Proteins, Membrane Proteins, Meta-Analysis as Topic, Minor Histocompatibility Antigens, Peptide Fragments, Polymorphism, Genetic, Receptors, Peptide, Receptors, Prostaglandin E, EP4 Subtype, Receptors, Tumor Necrosis Factor, Type I, Spondylitis, Ankylosing

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