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Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-γ Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3-21 months. 62% of RD1-Elispot negative individuals were positive by cultured Elispot. Fluctuations in T cell response were observed with rates of change ranging from -150 to +153 spot-forming cells (SFC)/200,000 PBMC in a 3-month period. To validate these responses we used an RD1-specific real time quantitative PCR assay for monokine-induced by IFN-γ (MIG) and IFN-γ inducible protein-10 (IP10) (MIG: r=0.6527, p=0.0114; IP-10: r=0.6967, p=0.0056; IP-10+MIG: r=0.7055, p=0.0048). During follow-up 30 individuals were placed on ARVs and 4 progressed to active TB. Fluctuations in SFC did not correlate with CD4 count, viral load, treatment initiation, or progression to active TB. The RD1-Elispot appears to have limited value in this setting.

Original publication

DOI

10.1371/journal.pone.0037920

Type

Journal article

Journal

PLoS One

Publication Date

2012

Volume

7

Keywords

CD4 Lymphocyte Count, Chemokine CXCL10, Chemokine CXCL9, Coinfection, Enzyme-Linked Immunospot Assay, HIV, HIV Infections, Humans, Limit of Detection, Mycobacterium tuberculosis, Prospective Studies, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Time Factors, Tuberculosis, Viral Load