Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Marginal zone (MZ) B cells are thought to be responsible for the first wave of Abs against bacterial Ags. In this study, we assessed the in vivo response of MZ B cells in mice immunized with viral particles derived from the RNA phage Qbeta. We found that both follicular (FO) and MZ B cells responded to immunization with viral particles. MZ B cells responded with slightly faster kinetics, but numerically, FO B cells dominated the response. B1 B cells responded similarly to MZ B cells. Both MZ and FO B cells underwent isotype switching, with MZ B cells again exhibiting faster kinetics. In fact, almost all Qbeta-specific MZ B cells expressed surface IgG by day 5. Histological analysis demonstrated that a population of activated B cells remain associated with the MZ, probably due to the elevated integrin levels expressed by these cells. Thus, both MZ and FO B cells respond with rapid proliferation to viral infection and both populations undergo isotype switching, but MZ B cells remain in the MZ and may be responsible for local Ab production, opsonizing pathogens entering the spleen.

Original publication

DOI

10.4049/jimmunol.173.7.4308

Type

Journal article

Journal

J Immunol

Publication Date

01/10/2004

Volume

173

Pages

4308 - 4316

Keywords

Allolevivirus, Animals, Antigens, CD, B-Lymphocyte Subsets, Biomarkers, Female, Flow Cytometry, Germinal Center, Immunoglobulin Class Switching, Immunoglobulin G, Immunoglobulin M, Integrin alpha4beta1, Lymphocyte Cooperation, Lymphocyte Function-Associated Antigen-1, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Receptors, Virus, Spleen, T-Lymphocytes, Helper-Inducer, Tetraspanin 29, Time Factors, Up-Regulation, Virion