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Neutralizing antibodies are necessary and sufficient for protection against infection with vesicular stomatitis virus (VSV). The in vitro neutralization capacities and in vivo protective capacities of a panel of immunoglobulin G monoclonal antibodies to the glycoprotein of VSV were evaluated. In vitro, neutralizing activity correlated with avidity and with neutralization rate constant, a measure of on-rate. However, in vivo, protection was independent of immunoglobulin subclass, avidity, neutralization rate constant, and in vitro neutralizing activity; above a minimal avidity threshold, protection depended simply on a minimum serum concentration. These two biologically defined thresholds of antibody specificity offer hope for the development of adoptive therapy with neutralizing antibodies.

Type

Journal article

Journal

Science

Publication Date

27/06/1997

Volume

276

Pages

2024 - 2027

Keywords

Animals, Antibodies, Monoclonal, Antibodies, Viral, Antibody Affinity, Antibody Specificity, Brain, Enzyme-Linked Immunosorbent Assay, Glycoproteins, Immunization, Passive, Immunoglobulin G, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, SCID, Neutralization Tests, Rhabdoviridae Infections, Vesicular stomatitis Indiana virus, Viral Envelope Proteins