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Sensitivities of in vitro and in vivo assays for the detection of lymphocytic choriomeningitis virus (LCMV)-specific CTL were compared. Measurement of primary cytotoxicity was the least sensitive of all the tested assays. However, when the same 51Cr release assays were performed after in vitro restimulation, this in vitro method was found to be more sensitive than all of the five in vivo assays tested. Assessment of CTL-mediated protection against LCMV replication in spleens was most sensitive among the in vivo tests, followed by CTL-mediated resistance to intracerebral or intraperitoneal challenge infections with vaccinia-LCMV-recombinant virus. Inhibition of LCMV-induced choriomeningitis and of the footpad swelling reaction were least sensitive in detecting LCMV-specific CTL. As discussed, the presented sensitivity gradient may most probably be generalized.

Original publication

DOI

10.1006/cimm.1993.1254

Type

Journal article

Journal

Cell Immunol

Publication Date

15/10/1993

Volume

151

Pages

460 - 466

Keywords

Animals, Cytotoxicity Tests, Immunologic, Edema, Female, Immunologic Memory, In Vitro Techniques, Lymphocytic Choriomeningitis, Lymphocytic choriomeningitis virus, Meninges, Mice, Mice, Inbred C57BL, Ovary, Sensitivity and Specificity, Spleen, T-Lymphocytes, Cytotoxic, Virus Replication