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Mammalian protein expression systems are becoming increasingly popular for the production of eukaryotic secreted and cell surface proteins. Here we describe methods to produce recombinant proteins in adherent or suspension human embryonic kidney cell cultures, using transient transfection or stable cell lines. The protocols are easy to scale up and cost-efficient, making them suitable for protein crystallization projects and other applications that require high protein yields.

Original publication

DOI

10.1007/978-1-4939-2230-7_6

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2015

Volume

1261

Pages

115 - 127

Keywords

Biotechnology, Cell Adhesion, Cell Culture Techniques, Glycoproteins, HEK293 Cells, Humans, Recombinant Proteins, Transfection