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ASPP family is proved to be apoptotic specific regulators of p53. Among them, iASPP acts as an inhibitor of p53. To investigate the effect of the iASPP RNAi on the apoptosis of breast cancer cell MCF-7, we transfected the recombinant plasmid PGCsilencer H1/Neo/GFP/RNAi into MCF-7. The iASPP expression was analyzed by RT-PCR and Western blot. The cell apoptosis was detected by FCM. The results show that the expression of iASPP is descended and the apoptosis rate is increased after transfection. Therefore, we conclude that inhibition of expression of iASPP may resume the ability of p53 to induce apoptosis in MCF-7 cells.

Original publication

DOI

10.1080/07357900801965042

Type

Journal article

Journal

Cancer Invest

Publication Date

11/2008

Volume

26

Pages

878 - 882

Keywords

Apoptosis, Breast Neoplasms, Cell Line, Tumor, Humans, Intracellular Signaling Peptides and Proteins, Plasmids, Protein Biosynthesis, RNA Interference, Repressor Proteins, Transfection, Tumor Suppressor Protein p53