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Using Streptococcus pneumoniae as an example, the ability of multilocus sequence typing (MLST) to characterise isolates directly from cerebrospinal fluid (CSF) was investigated. A nested multiplex polymerase chain reaction method that amplifies the seven housekeeping gene fragments used for pneumococcal MLST was applied to 30 CSF samples from suspected cases of bacterial meningitis. The fragments were amplified from all 14 samples from which Streptococcus pneumoniae was cultured, and, after direct sequencing, the allelic profiles obtained from ten of the samples corresponded to those of clones previously associated with invasive pneumococcal disease. MLST could also predict the penicillin susceptibility and serotype of the CSF isolates.

Type

Journal article

Journal

Eur J Clin Microbiol Infect Dis

Publication Date

08/2000

Volume

19

Pages

627 - 630

Keywords

Alleles, Bacteria, Bacterial Typing Techniques, Cerebrospinal Fluid, Humans, Meningitis, Bacterial, Meningitis, Pneumococcal, Neisseria meningitidis, Polymerase Chain Reaction, Serotyping, Streptococcus pneumoniae