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Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells. Methods used for the identification of spliced peptides on tumors are also described.

Original publication

DOI

10.1007/978-1-4939-9450-2_13

Type

Chapter

Publication Date

2019

Volume

1988

Pages

159 - 186

Keywords

Chromium release assay, Cytolytic T lymphocytes, Peptide identification, Spliced peptides, cDNA library