Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal β-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection using roller bottles. Two different purification approaches have been applied to obtain purified NS1 (sNS1) and the C-terminal β-ladder domain ready for clinical applications.

Original publication

DOI

10.1007/978-1-0716-0581-3_8

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/01/2020

Volume

2142

Pages

93 - 102

Keywords

Protein purification, NS1 glycoprotein, Protein production, Zika virus, HEK293T