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Primary cilia regulate and coordinate a variety of cell signaling pathways important in chondrocyte physiology and cartilage development, health, and disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.

Original publication

DOI

10.1007/978-1-0716-2839-3_12

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2023

Volume

2598

Pages

157 - 176

Keywords

Chondrocyte, Confocal microscopy, Immunofluorescence, Primary cilia, Quantification, Super resolution, Chondrocytes, Cartilage, Articular, Cilia, Signal Transduction