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Using oligonucleotide primers complementary to conserved regions in the mouse erythropoietin (Epo) gene, a portion of the rat Epo gene was amplified by the polymerase chain reaction to produce a probe suitable for assay of rat Epo mRNA by RNAse protection. The assay, which has sufficient sensitivity to measure to Epo mRNA in unstimulated rat kidneys, was used to demonstrate high amplitude in vitro modulation of Epo mRNA levels in response to changes in perfusate flow rate and oxygen tension in isolated kidneys, thus providing clear evidence that all the necessary events linking changes in oxygen delivery to the modulation of Epo mRNA levels can occur intrarenally.

Original publication

DOI

10.1084/jem.172.2.657

Type

Journal article

Journal

J Exp Med

Publication Date

01/08/1990

Volume

172

Pages

657 - 660

Keywords

Animals, Erythropoietin, Gene Expression Regulation, In Vitro Techniques, Kidney, Male, Oligonucleotide Probes, Oxygen, Partial Pressure, Perfusion, Polymerase Chain Reaction, RNA, Messenger, Rats, Rats, Inbred Strains, Ribonucleases, Transcription, Genetic