Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.
Bird LE., Rada H., Flanagan J., Diprose JM., Gilbert RJC., Owens RJ.
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.