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RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.

Original publication

DOI

10.1007/s13238-014-0076-6

Type

Journal article

Journal

Protein & cell

Publication Date

11/2014

Volume

5

Pages

805 - 815

Addresses

Department of Oncology, John Radcliffe Hospital, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK, shan.gao@oncology.ox.ac.uk.

Keywords

Animals, Humans, Virus Diseases, Neoplasms, Genetic Predisposition to Disease, Viral Proteins, RNA Interference, Molecular Targeted Therapy