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A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFNγ producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein.

Original publication

DOI

10.1016/j.jim.2014.10.012

Type

Journal article

Journal

J Immunol Methods

Publication Date

01/2015

Volume

416

Pages

40 - 48

Keywords

Adaptive antiviral responses, Antigen presentation, Avian influenza, CD8 T cell, IFNγ ELISpot, Animals, Antibodies, Viral, Antigens, Viral, CD8 Antigens, Cell Line, Chickens, Coculture Techniques, Dogs, Enzyme-Linked Immunospot Assay, Influenza in Birds, Interferon-gamma, Kidney, Madin Darby Canine Kidney Cells, T-Lymphocytes, Vaccination