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In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

Original publication

DOI

10.1128/aac.02169-16

Type

Journal article

Journal

Antimicrobial agents and chemotherapy

Publication Date

04/2017

Volume

61

Addresses

Public Health England West Midlands Public Health Laboratory, Heartlands Hospital, Birmingham, United Kingdom.

Keywords

Humans, Mycobacterium tuberculosis, Tuberculosis, Multidrug-Resistant, Fluoroquinolones, DNA Gyrase, Oligonucleotide Probes, Antitubercular Agents, False Positive Reactions, Biological Assay, Phylogeny, Drug Resistance, Multiple, Bacterial, Gene Expression, Mutation