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Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.

Original publication

DOI

10.1128/JVI.02261-13

Type

Journal article

Journal

J Virol

Publication Date

12/2013

Volume

87

Pages

12667 - 12674

Keywords

Gene Library, Genetic Engineering, RNA Viruses, RNA, Viral