Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Mutations in the leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson's disease, chronic inflammation and mycobacterial infections. Although there is evidence supporting the idea that LRRK2 has an immune function, the cellular function of this kinase is still largely unknown. By using genetic, pharmacological and proteomics approaches, we show that LRRK2 kinase activity negatively regulates phagosome maturation via the recruitment of the Class III phosphatidylinositol-3 kinase complex and Rubicon to the phagosome in macrophages. Moreover, inhibition of LRRK2 kinase activity in mouse and human macrophages enhanced Mycobacterium tuberculosis phagosome maturation and mycobacterial control independently of autophagy. In vivo, LRRK2 deficiency in mice resulted in a significant decrease in M. tuberculosis burdens early during the infection. Collectively, our findings provide a molecular mechanism explaining genetic evidence linking LRRK2 to mycobacterial diseases and establish an LRRK2-dependent cellular pathway that controls M. tuberculosis replication by regulating phagosome maturation.

Original publication

DOI

10.15252/embj.201798694

Type

Journal article

Journal

EMBO J

Publication Date

15/06/2018

Volume

37

Keywords

LRRK2, Parkinson's disease, Rubicon, phagosome, tuberculosis, Animals, Humans, Intracellular Signaling Peptides and Proteins, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2, Macrophages, Mice, Mice, Knockout, Mycobacterium tuberculosis, Phagosomes, Phosphatidylinositol 3-Kinases, Tuberculosis