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The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T11(2) and T11(3), PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 gamma-chain. The combination of T11(2) and T11(3) mAb also induced phosphorylation of the TCR zeta-chain. The phosphorylated zeta-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T11(2) and T11(3) mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 gamma-chain and the tyrosine phosphorylation of the zeta-chain, respectively.

Type

Journal article

Journal

J Immunol

Publication Date

01/02/1990

Volume

144

Pages

1010 - 1014

Keywords

Antigens, Differentiation, T-Lymphocyte, CD2 Antigens, CD3 Complex, Electrophoresis, Gel, Two-Dimensional, Enzyme Activation, Humans, In Vitro Techniques, Lymphocyte Activation, Phosphorylation, Phosphotyrosine, Protein-Tyrosine Kinases, Receptors, Antigen, T-Cell, Receptors, Immunologic, Signal Transduction, T-Lymphocytes, Tyrosine