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DNA vaccines encoding the human papillomavirus type-16 (HPV-16) E6 and E7 oncoproteins genetically fused to the human herpes simplex virus type 1 (HSV-1) gD protein were tested in mice for induction of T cell-mediated immunity and protection against tumor cell challenge. Hybrid genes, generated after insertion of E6 or E7-encoding sequences into internal sites of the gD-encoding gene, were transcribed in vitro and the chimeric proteins were expressed at the surface of in vitro-transfected mammalian cells. Female C57BL/6 mice immunized with 4 intramuscular doses (100 microg of DNA/dose) of the DNA vaccines encoding E7 efficiently generated E7-specific CD8(+) T cells. Vaccination of mice with the DNA vaccines encoding the E7, or both E6 and E7, conferred complete protection to challenges from TC-1 tumor cells and partial therapeutic effect (40%) in mice inoculated with TC-1 cells on the same day or 5 days prior to the first vaccine dose.

Original publication

DOI

10.1016/j.micinf.2005.05.024

Type

Journal article

Journal

Microbes Infect

Publication Date

12/2005

Volume

7

Pages

1541 - 1550

Keywords

Animals, CD8-Positive T-Lymphocytes, Cytokines, Cytoplasm, Disease Models, Animal, Female, Flow Cytometry, Herpesvirus 1, Human, Herpesvirus Vaccines, Human papillomavirus 16, Humans, Interferon-gamma, Membrane Proteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Neoplasms, Experimental, Oncogene Proteins, Viral, Papillomavirus E7 Proteins, Papillomavirus Infections, Papillomavirus Vaccines, Recombinant Fusion Proteins, Repressor Proteins, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines