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Single-particle electron cryomicrosopy permits structural characterization of noncrystalline protein, samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

Original publication




Journal article


Nano Letters

Publication Date





657 - 660


single-particle electron cryomicrosopy DNA templates nanostructure nanoaffinity g-protein-coupled membrane receptor coupled receptor crystal-structure electron cryomicroscopy neurotensin microscopy virus