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Single-particle electron cryomicrosopy permits structural characterization of noncrystalline protein, samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

Original publication

DOI

10.1021/Nl1037769

Type

Journal article

Journal

Nano Letters

Publication Date

2011

Volume

11

Pages

657 - 660

Keywords

single-particle electron cryomicrosopy DNA templates nanostructure nanoaffinity g-protein-coupled membrane receptor coupled receptor crystal-structure electron cryomicroscopy neurotensin microscopy virus