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DNA assembly methods are essential for multiple applications including synthetic biology. We recently developed MetClo, a method that uses a single type IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.

Original publication

DOI

10.1007/978-1-0716-0908-8_9

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2020

Volume

2205

Pages

143 - 159

Keywords

DNA assembly, MetClo, Modular assembly, Synthetic biology, Type IIS restriction enzyme