Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle.

Original publication

DOI

10.1074/jbc.M301041200

Type

Journal article

Journal

J Biol Chem

Publication Date

10/10/2003

Volume

278

Pages

40385 - 40391

Keywords

5' Untranslated Regions, Amino Acid Sequence, Base Sequence, Binding Sites, Gene Products, rev, Genes, env, HIV-1, In Vitro Techniques, Molecular Sequence Data, Mutagenesis, Nuclear Magnetic Resonance, Biomolecular, Nucleic Acid Conformation, RNA, Viral, Sequence Deletion, Surface Plasmon Resonance, rev Gene Products, Human Immunodeficiency Virus