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Cryo-EM is a powerful tool for understanding macromolecular structures, yet current methods for structure reconstruction are slow and computationally demanding. To accelerate research on pose estimation, we present CESPED, a data set specifically designed for supervised pose estimation in cryo-EM. Alongside CESPED, we provide a package to simplify cryo-EM data handling and model evaluation. We evaluate the performance of a baseline model, Image2Sphere, on CESPED, which shows promising results but also highlights the need for further improvements. Additionally, we illustrate the potential of deep learning-based pose estimators to generalize across different samples, suggesting a promising path toward more efficient processing strategies. Published by the American Physical Society 2024

Original publication

DOI

10.1103/physrevresearch.6.023245

Type

Journal article

Journal

Physical Review Research

Publisher

American Physical Society (APS)

Publication Date

04/06/2024

Volume

6