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L cells expressing either the A/NT/60/68 nucleoprotein or the A/PR/8/34 (H1) hemagglutinin by DNA mediated gene transfer were used to investigate recognition by influenza A specific cytotoxic T lymphocytes (CTL). A subpopulation of CTL that recognized the H1 hemagglutinin was detected in mice primed with either A/PR/8/34 (H1N1) or A/JAP/305/57 (H2N2) influenza viruses. However, neither CTL from mice primed with A/NT/60/68 (H3N2) nor the recombinant virus X31 (H3N2) showed any activity on L cells expressing H1. These results showed that the majority of fully crossreactive CTL do not recognize the hemagglutinin molecule. A comparison between nucleoprotein and hemagglutinin transfected L cells reveals the nucleoprotein as the major target for CTL that are crossreactive on the three pandemic strains of human influenza A virus.


Journal article



Publication Date





13 - 25


Animals, Cells, Cultured, DNA, DNA Restriction Enzymes, Female, Immunoenzyme Techniques, Influenza A virus, L Cells (Cell Line), Mice, Mice, Inbred Strains, Plasmids, Simian virus 40, T-Lymphocytes, Cytotoxic, Transcription, Genetic, Transfection, Viral Envelope Proteins, Viral Matrix Proteins, Viral Proteins