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We consider a realistic suicide substrate reaction which can be represented by four rate equations for the concentrations of the various molecules as functions of time. We present a general procedure to obtain accurate, approximate solutions analytically in terms of the rate equation parameters. This systematic technique provides more accurate approximations to the exact (numerical) solutions than other approximate methods which have been proposed based on a pseudo-steady state hypothesis.

Type

Journal article

Journal

Biophys Chem

Publication Date

31/08/1990

Volume

37

Pages

81 - 90

Keywords

Enzyme Inhibitors, Enzymes, Kinetics, Mathematics, Models, Theoretical, Protein Binding