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The administration of recombinant vaccinia virus Ankara (MVA) encoding a CTL epitope (pb9) from a malaria antigen induced activation and maturation of splenic dendritic cells (DCs) in vivo. In contrast, incubation of immature dendritic cells (iDCs) with the MVA, in vitro, resulted in down-regulation of MHC class I molecules and reduced their T-cell stimulatory ability. However, the ability of the infected DC to induce an antigen-specific CTL response, in vivo, remained intact. Furthermore, the administration of recombinant MVA-infected DC, but not pb9 peptide-pulsed DC, boosted and expanded the anti-pb9 CTL response that was primed by pb9 peptide-pulsed DC. These data indicate that despite the ability of poxviruses to impair DC maturation in vivo, the important ability of MVA to boost CD8 T-cell response in vivo is mediated at the level of the infected dendritic cells.

Original publication

DOI

10.1016/j.vaccine.2004.04.029

Type

Journal article

Journal

Vaccine

Publication Date

22/10/2004

Volume

22

Pages

4326 - 4331

Keywords

Animals, Bone Marrow, Cell Proliferation, Cell Separation, Dendritic Cells, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Genes, MHC Class I, Interferon-gamma, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Phenotype, Stimulation, Chemical, T-Lymphocytes, Cytotoxic, Vaccines, Attenuated, Vaccinia virus