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A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear.

Type

Journal article

Journal

Acta Virol

Publication Date

2002

Volume

46

Pages

117 - 120

Keywords

Animals, Cell Extracts, Cell Fractionation, Chromatography, Liquid, Dermacentor, Electrophoresis, Gel, Two-Dimensional, Female, Interleukin-8, L Cells (Cell Line), Mice, Nucleocapsid, Nucleocapsid Proteins, Salivary Glands, Vesicular stomatitis Indiana virus