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Dendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up-regulate co-stimulatory molecules, induce T-cell proliferative responses and secrete interleukin-12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T-cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte-derived human DC in vitro, but lipopolysaccharide-treated monocyte-derived DC showed enhanced functional activity and up-regulated co-stimulatory molecules.

Original publication




Journal article



Publication Date





361 - 366


Animals, Antigens, CD, B7-2 Antigen, CD40 Antigens, Cell Movement, Cells, Cultured, CpG Islands, Dendritic Cells, Female, Flow Cytometry, Histocompatibility Antigens Class II, Humans, Immunohistochemistry, Integrin alphaXbeta2, Interleukin-12, Lectins, C-Type, Lipopolysaccharides, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Minor Histocompatibility Antigens, Oligonucleotides, Receptors, Cell Surface, Spleen