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Human lymphokine-activated killer (LAK) cells were generated from peripheral blood lymphocytes (PBL) of normal volunteers by interleukin-2 (IL-2) stimulation for 1-8 days. During the first 3 days the surface marker CD16 characteristic for natural killer (NK) cells was expressed and later the CD3 marker characteristic for cytotoxic T cells became predominant. The conditioned media of LAK cells collected after interaction of LAK cells with K562 target cells was chromatographically separated into two cytotoxic fractions: F1 and F2. It was demonstrated that fraction F1 contained cytotoxic proteins having molecular weights of 30 and 40 kDa, and fraction F2 contained cytotoxic proteins having molecular weights of 22, 38 and 75 kDa. The presence of the proteins in each of these two fractions correlated with the phenotype changes of LAK cells: the F2 cytotoxic proteins were characteristic for NK-like cells, and the F1 proteins for cytotoxic T-lymphocyte (CTL)-like phenotypes.

Type

Journal article

Journal

Immunol Lett

Publication Date

08/1993

Volume

37

Pages

153 - 157

Keywords

CD3 Complex, Cells, Cultured, Chromatography, Ion Exchange, Culture Media, Electrophoresis, Polyacrylamide Gel, Humans, Immunophenotyping, Interleukin-2, Killer Cells, Lymphokine-Activated, Killer Cells, Natural, Killer Factors, Yeast, Lymphocyte Activation, Molecular Weight, Proteins, Receptors, IgG, T-Lymphocytes, Cytotoxic, Time Factors, Tumor Cells, Cultured