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Restriction endonuclease digestion of linear borrelial chromosomal DNA showed that three isolates of UK Lyme disease spirochaetes differed markedly from each other and from published data for other isolates from North America and continental Europe. Analysis of linear plasmid bands revealed that UK isolates differed from each other in the number and sizes of the plasmids in isolates from different foci of UK Lyme disease. Fatty acid analysis (of fatty acid methyl ester (FAME) profiles) showed the UK isolates clustering together with the relapsing fever spirochaetes, Borrelia turicatae and Borrelia parkeri. These data are discussed in respect of current knowledge of Lyme borreliosis in the UK.

Original publication

DOI

10.1111/j.1574-6968.1995.tb07712.x

Type

Journal article

Journal

FEMS Microbiol Lett

Publication Date

01/08/1995

Volume

130

Pages

151 - 157

Keywords

Borrelia burgdorferi Group, DNA, Bacterial, Fatty Acids, Lyme Disease, Plasmids, Restriction Mapping