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Infection with genotype 4 of the Hepatitis C virus is common in Africa and the Mediterranean area, but has also been found at increasing frequencies in injection drug users in Europe and North America. Full length viral sequences to characterize viral diversity and structure have recently become available mostly for subtype 4a, and studies in Egypt and Saudi Arabia, where high proportions of subtype 4a infected patients exist, have begun to establish optimized treatment regimens. However knowledge about other subtype variants of genotype 4 present in less developed African states is lacking. In this study the full coding region from so far poorly characterized variants of HCV genotype 4 was amplified and sequenced using a long range PCR technique. Sequences were analyzed with respect to phylogenetic relationship, possible recombination and prominent sequence characteristics compared to other known HCV strains. We present for the first time two full-length sequences from the HCV genotype 4k, in addition to five strains from HCV genotypes 4d and 4f. Reference sequences for accurate HCV genotyping are required for optimized treatment, and a better knowledge of the global viral sequence diversity is needed to guide vaccines or new drugs effective in the world wide epidemic.

Original publication

DOI

10.1002/jmv.21240

Type

Journal article

Journal

J Med Virol

Publication Date

08/2008

Volume

80

Pages

1370 - 1378

Keywords

Amino Acid Sequence, Base Sequence, Genotype, Hepacivirus, Hepatitis C, Humans, Molecular Sequence Data, Open Reading Frames, Phylogeny, Polymerase Chain Reaction, Recombination, Genetic, Reference Standards, Sequence Alignment, Sequence Analysis, DNA