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Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.

Original publication

DOI

10.1371/journal.pone.0184496

Type

Journal article

Journal

PloS one

Publication Date

11/10/2017

Volume

12

Pages

e0184496 - e0184496

Addresses

Harvard Medical School, Boston, Massachusetts, United States of America.

Keywords

CD8-Positive T-Lymphocytes, Humans, HIV Infections, Antiviral Agents, Lymphocyte Depletion, Endocytosis, Ribosome Inactivating Proteins, Type 1, Protein Multimerization