Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Plasmids containing sequences 3' of the adult beta 1 globin gene of Xenopus laevis are unstable on propagation in a range of E. coli host strains. Up to 300 bp of Xenopus DNA are lost by rec A independent recombination between (AT)37 and (AT)17 sequences. Additionally, smaller deletions occurring in or around the (AT)37 sequence are observed. Deletion of these potential cruciform structures occurs in the absence of exonuclease I, exonuclease V and exonuclease VIII as the same pattern of deletion events is observed in recA recBC sbcB and recBC sbcA recE strains.

Original publication




Journal article


Nucleic Acids Res

Publication Date





4147 - 4158


Animals, Base Sequence, Chromosome Deletion, DNA Restriction Enzymes, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Agar Gel, Escherichia coli, Globins, Nucleic Acid Hybridization, Plasmids, Recombination, Genetic, Xenopus laevis