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Immune cell development and function occur in specialized immunological tissues, the function of which requires active cell migration and interactions between hematopoietic cells and underlying networks of stromal cells. These cells provide a scaffold on which immune cell migrate, provide microenvironments for efficient antigen presentation, and provide signals required for immune cell recruitment and survival. Technical advances in imaging technologies including multiphoton microscopy and 3D tissue reconstructions are being combined with computational approaches to provide new insights into the process of cell migration and function in immunological tissues.

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3D imaging, Migration, Modeling, Multiphoton, Thymus, Animals, Antigen Presentation, Cell Movement, Hematopoiesis, Homeostasis, Humans, Microscopy, Tissue Engineering, Tissue Scaffolds