Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.

Original publication

DOI

10.1007/978-1-4939-7837-3_19

Type

Chapter

Publication Date

2018

Volume

1784

Pages

197 - 214

Keywords

Chemokines, Chemotaxis, Inflammation, Macrophages, Migration, Adaptive Immunity, Biological Assay, Cell Movement, Chemokines, CC, Chemokines, CXC, Chemotactic Factors, Chemotaxis, Humans, Immunity, Innate, Inflammation, Interleukin-1, Macrophages