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We have identified the major structural core proteins of Ty virus-like particles (Ty-VLPs) and shown that they are generated by proteolytic cleavage of the primary translation product of TYA, p1. This precursor protein is therefore functionally similar to the gag precursor of retroviruses. Cleavage is mediated by a Ty-encoded protease located at the 5' region of TYB and is accompanied by a change in particle morphology. p1 contains sufficient information for the assembly of a pre-Ty-VLP complex, which does not require the presence of either Ty protease or reverse transcriptase. The results indicate that the requirements and pathway of Ty-VLP formation reflect the initial stages of mammalian retroviral assembly and further support the idea of a common origin for Ty elements and retroviruses.

Type

Journal article

Journal

Cell

Publication Date

10/04/1987

Volume

49

Pages

111 - 119

Keywords

Amino Acid Sequence, Base Sequence, Chromosome Deletion, DNA Transposable Elements, Escherichia coli, Fungal Proteins, Genes, Genes, Fungal, Genetic Vectors, Mutation, Retroviridae, Retroviridae Proteins, Saccharomyces cerevisiae, Species Specificity, Structure-Activity Relationship