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The porA genes from serogroup A, subgroup III strains isolated in the People's Republic of China in 1966 and in 1984 and 1985 were amplified by PCR at an annealing temperature of 75 degrees C. The DNA sequences (5 strains) and the restriction patterns generated by MspI (14 strains) were identical, unlike the results reported by Peixuan et al. (Z. Peixuan, H. Xujing, and X. Li, J. Clin. Microbiol. 33:458-462, 1995). Furthermore, PCR products which were amplified at an annealing temperature of 60 degrees C, as described by Peixuan et al., were heterogeneous in our study.

Type

Journal article

Journal

J Clin Microbiol

Publication Date

06/1996

Volume

34

Pages

1548 - 1550

Keywords

Alleles, China, DNA, Bacterial, Genes, Bacterial, Humans, Meningitis, Meningococcal, Molecular Epidemiology, Molecular Sequence Data, Neisseria meningitidis, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Porins, Serotyping, Time Factors