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Extracts prepared from the salivary glands (SGE) of partially fed adult female Rhipicephalus appendiculatus ticks reduced the expression by human peripheral blood leukocytes 9PBLs) of lipopolysaccharide (LPS)-stimulated cytokine mRNA. Treatment with SGE had no obvious effect on cytokine mRNA production when compared with untreated PBLs. LPS treatment induced or increased mRNA production for IFN alpha, TNF-alpha, IL-1 alpha, IL-1 beta, IL-5, IL-6, IL-7 and IL-8. All the LPS-stimulated cytokine mRNAs were reduced when treated with a mixture of LPS and SGE. The results indicate the potential of ticks in modulating the cytokine network of their vertebrate hosts, possibly to facilitate blood feeding.

Type

Journal article

Journal

Exp Appl Acarol

Publication Date

11/1995

Volume

19

Pages

671 - 676

Keywords

Animals, Base Sequence, Cells, Cultured, Cytokines, DNA Primers, Female, Humans, Leukocytes, Mononuclear, Lipopolysaccharides, Mice, Molecular Sequence Data, RNA, Messenger, Salivary Glands, Ticks